Achieve Robust Genotype and Phenotype Screening Results with XDel Knockout Technology
Figure 1. A) Across 77 genes, the arrayed XDel library achieved an average Knockout Score of 75%. A total of 86 genes were transfected into U2OS-Cas9 cells via nucleofection. Nine of the 86 genes failed due to sequencing errors. B) Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.
XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.