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CRISPR Reagents

Gene Knockout Kits (GKO Kits)

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Knockout any human or mouse protein-coding gene with >80% efficiency using our advanced XDel design, guaranteed. Ships in as few as 5 days.
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Data

Gene Knockout Kits Provide Superior Editing Efficiency in iPSC, Immortalized, and Primary Cells and Consistent Protein Depletion Using the XDel Design
Gene Knockout Kits Provide Superior Editing Efficiency in iPSC, Immortalized, and Primary Cells and Consistent Protein Depletion Using the XDel Design
Figure 1. A) On-target editing efficiency of XDel multiple guide design (MGKO) (pink bars) vs their 3 respective single-guide RNAs (blue bars) across 7 endogenous target loci transfected in two immortalized (IMM) and two iPSC cell lines. Each transfection was performed in triplicate and data were analyzed via NGS by EditCo's proprietary software analysis. B) Western blot analyses for all 5 knockout target genes indicate a complete depletion of proteins across the 3 specified time points (days 7, 14, and 21) relative to the negative controls. Knockout cell pools for the target genes were generated by nucleofecting U2OS cells with multi-guide sgRNA and Cas9 (as RNPs). A negative control pool was also transfected for each target using non-targeting sgRNA.
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About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.