Nucleases, Control gRNA, and More

A simplified easy-to-use kit designed for users to optimize their transfection protocols and become familiar with EditCo's chemically modified sgRNAs and CRISPR/Cas9 technology.

This kit contains a positive control multi-guide sgRNA that can be used to optimize transfection parameters prior to knocking out your gene of interest. The positive control multi-guide sgRNA is composed of three guides that induce a fragment deletion in the human TRAC gene. This kit is meant to be used in conjunction with the Gene Knockout Kit, which also uses a multi-guide strategy.

This multi-guide positive control is a validated trio of gRNA targeting TRAC in human cell types. Use this control to determine optimal conditions for maximum editing efficiency in your multi-guide experiments involving our Arrayed CRISPR Libraries or Gene Knockout Kit. For the full kit of the positive control with the addition of sequencing and PCR primers as well as Cas9 nuclease, purchase the Transfection Optimization Kit.

This positive control is a validated gRNA sequence targeting CDC42BPB with editing efficiency of 80% - 95% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency in your cell models.

This positive control is a validated gRNA sequence targeting RELA with editing efficiency of 80% - 90% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency in your cell models.

A single guide RNA targeting Rosa26 that you can use to optimize transfection conditions in your cell line to achieve maximum editing efficiency. It is important to note that the mouse Positive Control contains only 1 sgRNA compared to your Mouse Gene Knockout Kit which contains between 1-3 sgRNAs. The Mouse Positive Control can assist in selecting the optimal CRISPR transfection conditions.

Non-targeting gRNA sequences are designed to not target any genomic location in the human & mouse species. This control generates a non-edited control when analyzing edited cells.

Non-targeting gRNA sequences are designed to not target any genomic location in the human & mouse species. This control generates a non-edited control when analyzing edited cells.

10 mM Tris, 1 mM EDTA, pH 8.0. For resuspending or diluting gRNA libraries.

For resuspending or diluting gRNA libraries.