SpCas9 Nuclease, Control gRNA, and More

These kits include positive controls, available in either XDel or single-guide format, that are useful for optimizing transfection conditions or gaining familiarity with CRISPR/Cas9 technology before targeting your gene of interest. Each kit contains everything needed for a complete CRISPR experiment, including controls, SpCas9 protein with 2NLS signals for editing via electroporation or lipid based transfection, and primers for PCR genotyping the edited site via PCR and ICE analysis through Sanger sequencing.

The Transfection Optimization Kit (TOK) is designed to be used alongside the Gene Knockout Kit to target your gene of interest, as both leverage the XDel strategy for efficient gene editing.

CRISPR positive controls are necessary to validate and monitor the efficiency of your CRISPR editing experiment. These positive controls are validated sgRNA sequences that have demonstrated high editing efficiency across different cell types and are available in both XDel design and single-guide format.

These controls are ideal for building and optimizing your assay, optimizing your experimental conditions, monitoring the gene editing efficiency across different cell lines or workflows, and troubleshooting. They can be used in any cell type, including immortalized, iPSC, and primary cells. Each control includes chemically modified synthetic sgRNA, provided at the yield noted above.

A negative control in a CRISPR-Cas9 experiment is used to ensure that observed effects are due to the intended genetic perturbation rather than artifacts from the experimental workflow (such as off-target effects, cell stress, or other non-specific influences). These non-targeting gRNA sequences are designed to not target any genomic location in the human & mouse species and therefore no editing event occurs with SpCas9. These controls are validated sequences that have been shown to have no effect on cell viability across different cell types and are available in a single-guide format.

These controls are essential in gene-editing experiments to compare the phenotype of cells treated with a gene-targeting guide RNA to those treated with a non-targeting guide RNA. They are commonly included in CRISPR screens to help identify potential false positives. Each control includes chemically modified synthetic sgRNA, provided at the yield noted above.

AAVS1 is a safe harbor site in the human genome,located in intron 1 of the PPP1R2C gene. Safe harbors are a predetermined location within a genome where genetic modifications can be inserted without disrupting critical genes or causing unwanted side effects. In this context, AAVS1 is a well-validated "safe harbor" often used as negative controls to ensure that observed phenotypes are due to the intended gene editing and not off-target effects or other non-specific events. Additionally, AAVS1 can serve as positive controls to validate gene editing efficiency. Editing in this site is known not to cause any adverse effects on cells. The available sgRNAs targeting this site have high editing efficiency across different cell types and include both XDel design and single-guide format.

These controls are essential for evaluating the baseline cellular response to SpCas9 cutting in the absence of a phenotypic readout. They serve as negative controls, helping to distinguish intended gene-editing effects from unintended functional changes in cells. AAVS1 controls, in particular, are widely used in CRISPR screening experiments, where phenotypic readouts are typically assessed. These can be used in any cell type including immortalized, iPSC and primary cells Each control includes chemically modified synthetic sgRNA, provided at the yield noted above.

Lethal controls are positive controls that cause a cell death phenotype. These controls generally will target essential genes that are required for cell growth, therefore, when the gene is knocked out, the cells do not survive. PLK1 is a key regulator of the cell cycle with a central role in mitosis. This control has demonstrated high editing efficiency and hinders cell growth across different cell types and are provided in the XDel format. PLK1 knockout cells undergo cell death within 3 days of transfection and can be easily detected using common dead cell dyes, such as propidium iodide.

This control is ideal for optimizing the experimental conditions of your gene editing experiment and validating the transfection efficiency since they provide a very clear phenotype easily assessed via microscopy or a simple cell viability assay. Each control includes chemically modified synthetic sgRNA, provided at the yield noted above.