Engineered Cells

Knockout Immortalized Cell Lines

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** Please contact your EditCo representative for academic pricing

Streamline your gene editing with tailored CRISPR knockout pools and clones. Assay-ready cells using our advanced XDel design for high performance, or single-guide design. No optimization needed. Choose your target and cell line, and we'll take care of the rest. *Please contact your EditCo representative for academic pricing
Learn More About Knockout Immortalized Cell LinesDownload our Information on Supplying Your Own CellsExplore More Data in XDel Application Note

Gene + Design

LRRK2

ENSG00000188906

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]

Design specifications
Guides designed
3
Cuts canonical
Yes
Coding seq. cut
98%–100%
CCDS cut
1 of 1
Earliest exon cut
Exon 1
Off-target genes
None
Gene Ontology
Molecular function
SNARE bindingmagnesium ion bindingactin bindingGTPase activityprotein kinase activityprotein serine/threonine kinase activity
Biological process
MAPK cascadeprotein phosphorylationendocytosisresponse to oxidative stressmitochondrion organizationendoplasmic reticulum organization
Cellular component
Golgi membraneextracellular regioncytoplasmmitochondrionmitochondrial outer membranemitochondrial inner membrane
Cell line + LRRK2

A-549

Homo sapiens (Human)CVCL_0023

Cancer cell line · Male · 58Y · lung

LRRK2 DepMap profileACH-000681

This line vs. all DepMap-profiled lines for LRRK2.

CRISPR gene effectimpact on cell growth
Not essential0.01
-0.700.75
CRISPR dependencyreliance on this gene
Low0.02
0.000.85
Transcript expression
Transcriptlog₂(TPM+1)
Cut by guides6
ENST00000298910.12canonicalCCDS2.09
ENST00000343742.60.77
ENST00000680235.10.07
ENST00000679360.10.06
ENST00000644108.10.04
ENST00000680790.10.00
Popular targets by cell line
Engineered cell lines

Data

XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)
XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)
Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.
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About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.