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Engineered Cells

Knockout Immortalized Cell Lines

Configure your cells

Fast, Reliable CRISPR Knockout Pools and Clones. Consistent knockouts you can trust leveraging our XDel multi-guide designs with 98% average editing efficiency. NGS-verified with QC report, assay ready cells shipped in as fast as 2 weeks. *Please contact your EditCo representative for academic pricing.
Learn More About Knockout Immortalized Cell LinesDownload our Information on Supplying Your Own CellsExplore More Data in XDel Application Note

Gene + Design

LRRK2

ENSG00000188906

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]

Design specifications
Guides designed
3
Cuts canonical
Yes
Coding seq. cut
98%–100%
CCDS cut
1 of 1
Earliest exon cut
Exon 1
Off-target genes
None
Gene Ontology
Molecular function
SNARE bindingmagnesium ion bindingactin bindingGTPase activityprotein kinase activityprotein serine/threonine kinase activity
Biological process
MAPK cascadeprotein phosphorylationendocytosisresponse to oxidative stressmitochondrion organizationendoplasmic reticulum organization
Cellular component
Golgi membraneextracellular regioncytoplasmmitochondrionmitochondrial outer membranemitochondrial inner membrane
Cell line + LRRK2

A-549

Homo sapiens (Human)CVCL_0023

Cancer cell line · Male · 58Y · lung

LRRK2 DepMap profileACH-000681

This line vs. all DepMap-profiled lines for LRRK2.

CRISPR gene effectimpact on cell growth
Not essential0.01
-0.700.75
CRISPR dependencyreliance on this gene
Low0.02
0.000.85
Transcript expression
Transcriptlog₂(TPM+1)Count
Cut by guides6
ENST00000298910.12canonicalCCDS2.091,605
ENST00000343742.60.77163
ENST00000680235.10.075
ENST00000679360.10.0619
ENST00000644108.10.041
ENST00000680790.10.000
Popular targets by cell line
Engineered cell lines
  • TP53 × HEK293
  • APOE × HMC3
  • CRBN × HEK293
  • EGFR × PC-9

Data

XDel Knockout Cells Enable gene-edited knockout cell pools and clones delivered in as fast as 2 Weeks (Pools) or as fast as 8 Weeks (Clones)
XDel Knockout Cells Enable gene-edited knockout cell pools and clones delivered in as fast as 2 Weeks (Pools) or as fast as 8 Weeks (Clones)
Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.
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About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.