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Engineered Cells

Knockout Immortalized Cell Lines

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Fast, Reliable CRISPR Knockout Pools and Clones. Consistent knockouts you can trust leveraging our XDel multi-guide designs with 98% average editing efficiency. NGS-verified with QC report, assay ready cells shipped in as fast as 2 weeks. *Please contact your EditCo representative for academic pricing.
Learn More About Knockout Immortalized Cell LinesDownload our Information on Supplying Your Own CellsExplore More Data in XDel Application Note

Gene + Design

EGFR

ENSG00000146648

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020]

Design specifications
Guides designed
3
Cuts canonical
Yes
Coding seq. cut
90%–93%
CCDS cut
5 of 5
Earliest exon cut
Exon 3
Off-target genes
None
Gene Ontology
Molecular function
chromatin bindingdouble-stranded DNA bindingprotein kinase activityMAP kinase kinase kinase activityprotein tyrosine kinase activitytransmembrane receptor protein tyrosine kinase activity
Biological process
MAPK cascadeossificationpositive regulation of protein phosphorylationsignal transductioncell surface receptor signaling pathwaycell surface receptor protein tyrosine kinase signaling pathway
Cellular component
Golgi membraneextracellular regionnucleuscytoplasmendosomeendoplasmic reticulum membrane
Cell line + EGFR

PC-9

Homo sapiens (Human)CVCL_B260

Cancer cell line · Male · 45Y · lymph node

EGFR DepMap profileACH-000779
Transcript expression
Transcriptlog₂(TPM+1)Count
Cut by guides5
ENST00000275493.7canonicalCCDS6.8073,658
ENST00000344576.7CCDS0.5493
ENST00000455089.5CCDS0.4399
ENST00000420316.6CCDS0.2419
ENST00000342916.7CCDS0.000
Popular targets by cell line
Engineered cell lines
  • TP53 × HEK293
  • APOE × HMC3
  • CRBN × HEK293
  • LRRK2 × A549

Data

XDel Knockout Cells Enable gene-edited knockout cell pools and clones delivered in as fast as 2 Weeks (Pools) or as fast as 8 Weeks (Clones)
XDel Knockout Cells Enable gene-edited knockout cell pools and clones delivered in as fast as 2 Weeks (Pools) or as fast as 8 Weeks (Clones)
Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.
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About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.