Engineered Cells

Knockout Immortalized Cell Lines

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** Please contact your EditCo representative for academic pricing

Streamline your gene editing with tailored CRISPR knockout pools and clones. Assay-ready cells using our advanced XDel design for high performance, or single-guide design. No optimization needed. Choose your target and cell line, and we'll take care of the rest. *Please contact your EditCo representative for academic pricing
Learn More About Knockout Immortalized Cell LinesDownload our Information on Supplying Your Own CellsExplore More Data in XDel Application Note

Gene + Design

APOE

ENSG00000130203

The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]

Design specifications
Guides designed
3
Cuts canonical
Yes
Coding seq. cut
62%–75%
CCDS cut
1 of 1
Earliest exon cut
Exon 4
Off-target genes
None
Gene Ontology
Molecular function
amyloid-beta bindingsignaling receptor bindingstructural molecule activitylipid carrier activityprotein bindingphospholipid binding
Biological process
response to reactive oxygen speciesretinoid metabolic processnegative regulation of endothelial cell proliferationtriglyceride metabolic processlipid transportreceptor-mediated endocytosis
Cellular component
extracellular regionnucleuscytoplasmearly endosomemultivesicular bodyendoplasmic reticulum
Cell line + APOE

HMC3

Homo sapiens (Human)CVCL_II76

Transformed cell line · Sex unspecified · 8-10FW · future brain

APOE DepMap profile

No exact DepMap match for this line, so per-gene dependency/expression isn't available (only ~⅔ of lines are profiled).

Popular targets by cell line
Engineered cell lines

Data

XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)
XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)
Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.
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About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.