Engineered Cells

Knockout Immortalized Cell Lines

Configure your cells

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** Please contact your EditCo representative for academic pricing

Streamline your gene editing with tailored CRISPR knockout pools and clones. Assay-ready cells using our advanced XDel design for high performance, or single-guide design. No optimization needed. Choose your target and cell line, and we'll take care of the rest. *Please contact your EditCo representative for academic pricing
Learn More About Knockout Immortalized Cell LinesDownload our Information on Supplying Your Own CellsExplore More Data in XDel Application Note

Gene + Design

TP53

ENSG00000141510

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Design specifications
Guides designed
3
Cuts canonical
Yes
Coding seq. cut
54%–66%
CCDS cut
15 of 15
Earliest exon cut
Exon 5
Off-target genes
None
Gene Ontology
Molecular function
transcription cis-regulatory region bindingRNA polymerase II cis-regulatory region sequence-specific DNA bindingDNA-binding transcription factor activity, RNA polymerase II-specificcis-regulatory region sequence-specific DNA bindingcore promoter sequence-specific DNA bindingTFIID-class transcription factor complex binding
Biological process
negative regulation of transcription by RNA polymerase IIhematopoietic progenitor cell differentiationnucleotide-excision repairregulation of DNA-templated transcriptionregulation of transcription by RNA polymerase IIautophagy
Cellular component
chromatinnucleusnucleoplasmtranscription regulator complexnucleoluscytoplasm
Cell line + TP53

HEK293

Homo sapiens (Human)CVCL_0045

Transformed cell line · Female · Fetus · kidney

TP53 DepMap profile

No exact DepMap match for this line, so per-gene dependency/expression isn't available (only ~⅔ of lines are profiled).

Popular targets by cell line
Engineered cell lines

Data

XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)
XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)
Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.
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About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.