Engineered Cells

Knockout Immortalized Cell Lines

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** Please contact your EditCo representative for academic pricing

Streamline your gene editing with tailored CRISPR knockout pools and clones. Assay-ready cells using our advanced XDel design for high performance, or single-guide design. No optimization needed. Choose your target and cell line, and we'll take care of the rest. *Please contact your EditCo representative for academic pricing
Learn More About Knockout Immortalized Cell LinesDownload our Information on Supplying Your Own CellsExplore More Data in XDel Application Note

Gene + Design

CRBN

ENSG00000113851

This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Design specifications
Guides designed
3
Cuts canonical
Yes
Coding seq. cut
76%–86%
CCDS cut
2 of 2
Earliest exon cut
Exon 3
Off-target genes
None
Gene Ontology
Molecular function
protein bindingtransmembrane transporter binding
Biological process
protein ubiquitinationpositive regulation of Wnt signaling pathwaynegative regulation of protein-containing complex assemblypositive regulation of protein-containing complex assemblynegative regulation of monoatomic ion transmembrane transportlocomotory exploration behavior
Cellular component
nucleuscytoplasmcytosolmembraneCul4A-RING E3 ubiquitin ligase complexperinuclear region of cytoplasm
Cell line + CRBN

HEK293

Homo sapiens (Human)CVCL_0045

Transformed cell line · Female · Fetus · kidney

CRBN DepMap profile

No exact DepMap match for this line, so per-gene dependency/expression isn't available (only ~⅔ of lines are profiled).

Popular targets by cell line
Engineered cell lines

Data

XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)
XDel Knockout Cells Enable gene-edited knockout cell pools and clones - Guaranteed and Delivered in 4 Weeks (Pools) or 10 Weeks (Clones)
Figure 1. A) Four membrane proteins were knocked out in K562 cells using XDel technology, following EditCo Bio’s proprietary workflow. Protein levels were assessed via flow cytometry at four time points: days 3, 7, 14, and 21 post-transfection, and compared to negative controls (non-edited cells or cells electroporated with Cas9 only). Membrane protein depletion remained stable over time, and cell viability was consistently high (>90%) across all samples. B) Representative flow cytometry plots showing protein levels for CD46 and CD59 in XDel knockout pools at 21 days post-editing.
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About XDel Technology

XDel is a cutting-edge CRISPR knockout technology designed to remove the guesswork from gene editing. By leveraging an innovative guide RNA design strategy, XDel delivers highly efficient, consistent, and reproducible gene knockouts—empowering researchers to accelerate their discoveries with confidence.